2009, discovery of a brand new phenotype for microbes in slender nanofluidic slits; and first detection of local protein structures along DNA using strong-state nanopores
Reconstitution of ultrawide DNA origami pores in liposomes for transmembrane transport of macromolecules
[Laughs] ‘It was definitely a great risk! At some time, I had been very well informed that it could go Mistaken and that I could turn into a lot less effective, but I just ignored that. Curiosity has always been my strongest driving drive. And by chance all of it worked out.’
Our do the job was promptly followed up by Other people, most notably by Phaedon Avouris and colleagues at IBM, who in Oct 1998 revealed their results on transistors created from single- and multi-walled carbon nanotubes in Utilized Physics Letters.
2010, formulated a whole new way (‘wedging transfer’) to control nanostructures; initial report of DNA translocation via graphene nanopores; and understood hybrid nanopores by directed insertion of α-hemolysin into good-condition nanopores
The coiled-coil of your human Rad50 DNA restore protein has unique segments of improved overall flexibility
‘At the outset we checked out Organic devices that has a Actual physical watch and acted on The idea with the instruments we understood. It took a several years in advance of we started off Performing additional on the large Organic troubles. I then started to get in touch with it nanobiology, a term that did not exist at some time.
Dynamic ParB–DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation
Knowledge essential procedures regulating the spatial and dynamic organisation of your DNA in Are living cells.
Quite cool, but that wasn’t actually where by my interest lay. I wished to pioneer And that i experienced the feeling that was no more probable, because the most exciting essential insights experienced currently been uncovered. But my discipline experienced developed a lot of remarkable new resources to measure specific molecules. I made a decision which i wanted to apply this to biology, to take a look at it from a Bodily viewpoint.’
Due to the fact 2000 he moved to single-molecule biophysics and nanobiology, with investigate from reports of DNA loop extrusion and supercoiling to DNA translocation via nanopores. Extra just lately his analysis has centered on learning chromatin composition and cell division with micro organism on chip, whilst he is usually trying to finally Construct artificial cells from the bottom up.
Afterwards we spoke again briefly simply because Dekker felt that our discussion of his faith swiftly grew to become purely philosophical, whereas In addition, it concretely will help him in his operate.
One example is, we look at the nucleopore elaborate, a gateway amongst the cell nucleus plus the cytoplasm. This is a very sophisticated technique and therefore challenging to study in a very cell, so we use biomimetics. We reconstruct holes and afterwards insert personal features in the nucleopore complex to review transportation via it at The only-molecule amount.’
2004, discovery of new physics in translocation of DNA by means of nanopores; initially experimental study of ions conduction in nanofluidic channels; initial electrochemistry with particular person one-wall carbon nanotubes; STM detection and cees dekker Charge of phonons in carbon nanotubes; initial electrical docking of microtubules on kinesin-coated nanostructures; 1st biophysics characterization with the mechanical Attributes of double-stranded RNA; and initial solitary-molecule examine of DNA translocation by a restriction-modification enzyme.